Quantifying the Cytotoxicity of <em>Staphyloccus aureus</em> Against Human Polymorphonuclear Leukocytes
نویسندگان
چکیده
منابع مشابه
Human Polymorphonuclear Leukocytes
A B S T R A C T Acetyl glyceryl ether phosphorylcholine (AGEPC) and leukotriene B4 (LTB4) induce concentration-dependent neutrophil aggregation. On a molar basis, LTB4 is 10 to 100 times more potent than AGEPC. AGEPC-induced aggregation is attenuated by two inhibitors of arachidonate lipoxygenation, eicosatetraynoic acid and nordihydroguaiaretic acid, and to a lesser extent by the cyclooxygenas...
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OHARA, M., SHIRADO, M., MIYATA, M., SAKUMA, H., NANAMIYA, M., YOSHIDA, N., YOSHIDA, H. and KASUKAWA, R. Natural and Antibody-Dependent Cellular Cytotoxicity o f Polymorphonuclear Luekocytes. Tohoku J. exp. Med., 1983, 140 (1), 59-66 Cytotoxic activity of polymorphonuclear leukocytes (PMN) in the peripheral blood of patients with various diseases was demonstrated to K562 cells (natural cytotoxic...
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Enucleated human polymorphonuclear leukocytes (PMN) were prepared by centrifuging isolated, intact PMN over a discontinuous Ficoll gradient that contained 20 microM cytochalasin B. The enucleated cells (PMN cytoplasts) contained about one-third of the plasma membrane and about one-half of the cytoplasm present in intact PMN. The PMN cytoplasts contained no nucleus and hardly any granules. The v...
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Nine polypeptide peaks with antibiotic activity were resolved from human polymorphonuclear leukocyte azurophil granule membranes. All but 1 of the 12 constituent polypeptides were identified by N-terminal sequence analysis. Near quantitative recovery of protein and activity permitted an assessment of the contribution of each species to the overall respiratory-burst-independent antimicrobial cap...
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The effect of phorbol myristate acetate (PMA) on the antibody-dependent cellular cytotoxicity (ADCC) of human polymorphonuclear leukocytes (PMNs) against human erythroleukemic K562 cells was studied by the use of a 3-h 51Cr-release assay. Pretreatment of PMNs with PMA (10 ng/ml) for 60 min resulted in inhibition of subsequent ADCC. This inhibition was dependent on doses of PMA. The effect of pr...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2020
ISSN: 1940-087X
DOI: 10.3791/60681